Molecular Surface Interaction Network Laboratory
The Molecular Surface Interaction Network Lab houses molecular surface interaction equipment comprising surface plasmon resonance and quartz crystal microbalance with dissipation monitoring. These instruments facilitate analyses of how materials, biological molecules and cells interact on the nanoscale including protein-protein/peptide/antibody/DNA/carbohydrate, protein-material, material-cell interactions.
Instruments and Capability
Surface plasmon resonance is an optical technique that enables label-free real time detection of protein/peptide/antibody/nucleic acid/virus interactions and is widely used in industry for drug and antibody development and epitope mapping studies. This instrument provides kinetics, stoichiometry and affinity measures. The systems have 4 channels and are capable of auto-sampling allowing multiplexing of experiments for high throughput. The BiaCore 2000 is optimised for a broad range of molecular interactions while the BiaCore S200 is specialised for small molecule interactions (housed in SOMS).
Quartz crystal microbalance with dissipation monitoring is a gravimetric technique that allows label-free, real time detection of interactions between surface immobilised materials, DNA, lipids, nanoparticles, molecules or cells and these same entities in solution providing kinetics, stoichiometry and viscoelastic measurements of the binding events with ng/cm2 resolution. The Q-Sense Analyzer system has 4 channels allowing multiplexing of experiments for high throughput and is equipped with the electrochemical module allowing simultaneous electrochemical impedance spectroscopy measurements and a cell module enabling simultaneous microscopy imaging.
- Advice on instrument selection
- Advice on experimental design
- Training to independently use the instruments
- Training to analyse, model, present and interpret data
- Assistance with data analysis, presentation and interpretation for publication
- Option for staff operated data acquisition, analysis and interpretation
- Grant costing
Location and access
The Molecular Surface Interaction Network Lab is located in Building E26, level 2, room 2002 within the Bioanalytical Mass Spectrometry facility of the Mark Wainwright Analytical Centre.
Users are inducted into the Network Lab and provided with swipe-card access (Mon-Fri, 8am – 6pm) to the facility and access to the ACLS online instrument booking system .Once instrument training is complete. Experienced users are able to apply for continuous instrument and facility access.
Once users are trained to independently operate the instruments, research groups are charged $30 per hour capped at $600 per year for user operated access to the instruments. This fee supports the servicing requirements of the instruments, buffers to operate and maintain the instruments as well as the purchase of routinely used chips for users to trial new experiments.
Network Lab staff operation of the instruments, data analysis and interpretation are each charged at $80 per hour while automated operation of the instrument after set up by staff (BiaCore only) is charged at $20 per hour.
Prospective users are invited to contact the Network Lab Manager, Dr Zehra Elgundi, and Director, A/Prof Megan Lord, to discuss their experimental requirements and arrange instrument training.